Virus:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
Recombinant:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
Lysis:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
Staining:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
Flow Cytometry:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
Clinical Proteomics:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
Membrane:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
Fluorescence:Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse Ly-6C TotalSeq Biolegend Cat# 128051; RRID:AB_2832461 Anti-mouse I-A/I-E TotalSeq Biolegend Cat# 107659; RRID:AB_2832368 Ati-mouse H2-Kb/SIINFEKL TotalSeq Biolegend Cat# 141617; RRID:AB_2876484 APC Streptavidin BD Cat# 554067; RRID:AB_10050396 Annexin-V APC Biolegend Cat# 640920 Bacterial and virus strains E. coli JM109 competent cells Takara Bio Cat#9052 Chemicals, peptides, and recombinant proteins RPMI 1640 with glutamine Nacalai Tesque Cat# 30264-56 PBS Nacalai Tesque Cat# 14249-24 Fetal Bovine Serum Sima-Aldrich Cat# 173012 Pep-Strep Thermo Fisher Scientific Cat# 15070063 2-ME Thermo Fisher Scientific Cat# 21985023 HEPES Nacalai Tesque Cat# 1755794 ACK lysis buffer GIBCO Cat# A1049201 Tween 20 BIO.RAD Cat# 1610781B02 Tumor Tissue Dissociation Reagent BD Cat# 661563 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat#130-096-730 Anti-Biotin MicroBeads Miltenyi Biotec Cat# 130-090-485 EDTA Nacalai Tesqu Cat# 0689485 DMSO FUJIFILM WAKO Cat# 048-21985 OPTI-MEM Thermo Fisher Scientific Cat# 11058021 MEMα FUJIFILM WAKO Cat# 135-15175 human IL-2 Shionogi & Co. Ltd Cat# 058-69790-0 OVA peptide SIINFEKL GenScript N/A SV40 Large T antigen peptide SAINNYAQKL GenScript N/A Adpgk peptide ASMTNMELM GenScript N/A puromycin InvivoGen Cat# ant-pr-1 LIVE/DEAD Fixable Violet Dead cell stain kit Thermo Fisher Scientific Cat# L34955 LIVE/DEAD Fixable Aqua Dead cell stain kit Thermo Fisher Scientific Cat# L34957 DFO [Deferoxamine mesylate, Iron chelator] ABCAM Cat# ab120727 VH298 Selleck Cat# S8449 DOX [Doxycycline] Clontech Cat# Z1311N CFSE [CellTrace CFSE Cell Proliferation Kit, for flow cytometry] Thermo Fisher Scientific Cat# C34554 CellMask Plasma Membrane stains green Thermo Fisher Scientific Cat# C37608 BlockAce KAC Cat# UKB80 periodate-lysine-paraformaldehyde solution Muto Pure chemicals Cat# 33121 DAPI Biolegend Cat# 422801 Dako Fluorescence Mounting Medium Agilent Technologies Cat# S3023 Opal 520 reagent pack AKOYA Biosciences Cat# FP1487001KT Opal 540 reagent pack AKOYA Biosciences Cat# FP1494001KT Opal 570 reagent pack AKOYA Biosciences Cat# FP1488001KT Opal 620 reagent pack AKOYA Biosciences Cat# FP1495001KT Opal 690 reagent pack AKOYA Biosciences Cat# FP1497001KT (Continued on next page) Cell Reports 44, 115800, June 24, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opal Diluent AKOYA Biosciences Cat# FP1498 Glycine FUJIFILM WAKO Cat# 077-00735 EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Scientific Cat#21335 Recombinant mouse IFN-β Biolegend Cat# 581302 Mouse IFN-gamma DuoSet ELISA R&D systems Cat# DY485-05 Critical commercial assays Chromium Next GEM Single Cell 5 ′ kit v2, 16 rxns 10x Genomics Cat# PN-1000263 5 ′ Feature Barcode kit, 16 rxns 10x Genomics Cat# PN-1000256 Chromium Next GEM Chip K Single Cell kit, 48 rxns 10x Genomics Cat# PN-1000286 Dual Index kit TT Set A, 96 rxns 10x Genomics Cat# PN-1000215 Dual Index kit TN Set A, 96 rxns 10x Genomics Cat# PN-1000250 SPRIselect Reagent Kit Beckman Coulter B23318 KAPA Library Quantification kit KAPA Biosystems KK4854 Deposited data Sc-RNA seq and CITE-seq data This paper DDBJ ID: PRJDB20533 Experimental models: Cell lines RAW264 RIKEN-BRC (Tsukuba, JAPAN) RCB0535 RAW264-CH25H-OE This paper N/A RAW264-CH25H-KO This paper N/A RAW264-HIF1α-KO This paper N/A RAW264-TetOn-VHL This paper N/A RAW264-CH25HKO-TetOn-VHL This paper N/A MC38 Dr. Lotze MT (University of Pittsburgh) N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-B2MKO This paper N/A MC38-GFP This paper N/A MC38-OVA-GFP This paper N/A MC38-OVA-GFP-β2MKO This paper N/A EL4 Dr. Steinman RM (Rockefeller University) N/A E0771 CH3 BioSystems (Amherst, NY, USA) 94A001 CT26.WT ATCC CRL-2638 CMG14-12 Dr. Tanaka M (The Tokyo University of Pharmacy and Life Sciences) N/A Experimental models: Organisms/strains Mouse: C57BL/6 Charles River (Japan) Strain #000664 Mouse: Ly5.1 congenic RIKEN Animal Facility Strain #002014 Mouse: OT-1 TCR Tg Dr. Heath WR (The University of Melbourne) N/A Mouse: B6.129P2-Lyzs tm1(cre)Ifo/J RIKEN Animal Facility Strain #004781 Mouse: B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J Jackson Laboratory Strain #005236 Mouse: B6.129S2-Cd8a tm1Mak/J Jackson Laboratory Strain #002665 Mouse: B6(Cg)-Irf8 tm1.1Hm/J Jackson Laboratory Strain#:014175 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze (Ai14) Jackson Laboratory Strain #007914 Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J Jackson Laboratory Strain #008068 (Continued on next page) 18 Cell Reports 44, 115800, June 24, 2025
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